Structure of human Cdc45 and implications for CMG helicase function
نویسندگان
چکیده
منابع مشابه
Structure of human Cdc45 and implications for CMG helicase function
Cell division cycle protein 45 (Cdc45) is required for DNA synthesis during genome duplication, as a component of the Cdc45-MCM-GINS (CMG) helicase. Despite its essential biological function, its biochemical role in DNA replication has remained elusive. Here we report the 2.1-Å crystal structure of human Cdc45, which confirms its evolutionary link with the bacterial RecJ nuclease and reveals se...
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Chromosome transmission fidelity 4 (Ctf4) is a conserved protein required for DNA replication. In this report, interactions between human Ctf4 (hCtf4) and the replicative helicase containing the cell division cycle 45 (Cdc45)/minichromosome maintenance 2-7 (Mcm2-7)/Go, Ichi, Nii, and San (GINS) (CMG) proteins [human CMG (hCMG) complex] were examined. The hCtf4-CMG complex was isolated following...
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In eukaryotes, two Mcm2–7 rings are loaded onto DNA at origins of replication during G1 phase1,2. The double hexamer is inactive as a heli case but is activated upon entry into S phase by the Dbf4dependent Cdc7 kinase, cyclindependent kinase and several transient factors that activate origins; subsequently, Cdc45 and GINS assemble onto each Mcm2–7 complex, thereby forming two active CMG heli...
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ژورنال
عنوان ژورنال: Nature Communications
سال: 2016
ISSN: 2041-1723
DOI: 10.1038/ncomms11638